- 询价
- Absin
- abs135661
- 上海
- 2025年08月26日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Rabbit anti-ILK Polyclonal mAb
- 抗体英文名：
Rabbit anti-ILK Polyclonal Antibody
- 靶点：
见优宁维官网
- 浓度：
1mg/ml
- 宿主：
Rabbit
- 适应物种：
见优宁维官网
- 保质期：
见优宁维官网
- 抗原来源：
见优宁维官网
- 级别：
优级
- 库存：
999
- 供应商：
Absin
- 标记物：
见优宁维官网
- 克隆性：
见优宁维官网
- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 免疫原：
A synthesized peptide derived from Human ILK(Accession Q13418), corresponding to amino acid residues N430-K452.
- 规格：
50ug///100ug
别名：兔抗ILK多克隆抗体A synthesized peptide derived from Human ILK(Accession Q13418), corresponding to amino acid residues N430-K452. WB 1:500-1:1000, IHC 1:50-1:100, IF 1:100-400, ELISA(peptide) 1:20000-1:40000 , ELISA(peptide) 1:20000-1:40000Store at -20 °C for one year. Avoid repeated freeze/thaw cyclesWB,IHC,IF,ELISA见优宁维官网RabbitHuman;Mouse;Rat
克隆性：见优宁维官网
产品描述：
Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2).
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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