- 询价
- Absin
- abs135610
- 上海
- 2025年08月26日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Rabbit anti-p27 Polyclonal mAb
- 抗体英文名：
Rabbit anti-p27 Polyclonal Antibody
- 靶点：
见优宁维官网
- 浓度：
1mg/ml
- 宿主：
Rabbit
- 适应物种：
见优宁维官网
- 保质期：
见优宁维官网
- 抗原来源：
见优宁维官网
- 级别：
优级
- 库存：
999
- 供应商：
Absin
- 标记物：
见优宁维官网
- 克隆性：
见优宁维官网
- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 免疫原：
A synthesized peptide derived from Human CDKN1B(Accession P46527), corresponding to amino acid residues L144-R169.
- 规格：
100ug///50ug
别名：兔抗p27多克隆抗体A synthesized peptide derived from Human CDKN1B(Accession P46527), corresponding to amino acid residues L144-R169.WB: 1:200-1:500, IHC: 1:50-1:200, IF: 1:100-1:500, ICC: 1:100-1:500, ELISA: 1:20000-1:40000Store at -20 °C for one year. Avoid repeated freeze/thaw cyclesElisa,FlowCytometry,ICC,ICC-IF,IF,IHC,WB,other见优宁维官网RabbitHuman;Mouse;Rat
克隆性：见优宁维官网
产品描述：
p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin 2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).
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文献和实验then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
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