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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Rabbit anti-SIRT2 Polyclonal mAb
- 抗体英文名:
Rabbit anti-SIRT2 Polyclonal Antibody
- 靶点:
见优宁维官网
- 浓度:
1mg/ml
- 宿主:
Rabbit
- 适应物种:
见优宁维官网
- 保质期:
见优宁维官网
- 抗原来源:
见优宁维官网
- 级别:
优级
- 库存:
999
- 供应商:
Absin
- 标记物:
见优宁维官网
- 克隆性:
见优宁维官网
- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
- 免疫原:
A synthesized peptide derived from Human SIRT2(Accession Q8IXJ6), corresponding to amino acid residues A330-A358.
- 规格:
50ug///100ug
别名:兔抗SIRT2多克隆抗体A synthesized peptide derived from Human SIRT2(Accession Q8IXJ6), corresponding to amino acid residues A330-A358. WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000Store at -20 °C for one year. Avoid repeated freeze/thaw cyclesWB,IHC,ICC-IF,ELISA见优宁维官网RabbitHuman;Mouse;Rat
克隆性:见优宁维官网
产品描述:
Sirtuins are members of the NAD-dependent histone deacetylase family of proteins that participate in a variety of cellular functions, including histone deacetylation, gene silencing, chromosomal stability, and aging. SIRT2, a human homolog of the yeast SIR2 (silent information regulator-2), functions as transcriptional silencing mediator at mating-type loci, telomeres and ribosomal gene clusters. SIRT2 expression increases dramatically during mitosis and is multiply phosphorylated at the G(2)/M transition of the cell cycle. SIRT2 is part of a phosphorylation cascade where it is phosphorylated late in G(2), during M, and into the period of cytokinesis. Inhibition of SIRT2 is reported to rescue alpha-synuclein toxicity and modify inclusion morphology in a cellular model of Parkinson‘s disease (1-4).
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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