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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
【干冰】Mycoplasma TaqMan Probe/Primer and Control Set
- 供应商:
广州市左克
- 规格:
100Reactions
产品简介:
品牌:Norgen
货号:NGB-TM33110
产品名称:【干冰】Mycoplasma TaqMan Probe/Primer and Control Set
产品规格:100Reactions
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1)所有产品仅可用于科研目的的生物学实验(forresearchuseonly),严禁用于人体或动物的医疗目的
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文献和实验Detection of Mycoplasma by Culture
of mycoplasma colonies (see Figure 14 ). Criteria for a Valid Result All positive control agar plates and broths show evidence of mycoplasma by typical colony formation on agar plates and usually a color change in broths. All negative control
Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
(cell side down) onto slide. Keep slide covered with aluminum foil (Prod. No. Z18514-0 ), allowing it to set for at least 15 min at 37ºC or for 30 min at room temperature. Observe slide under uv Epi-Fluorescence at x1000. Notes
之mycoplasma (e.g. M. hyorhinis)。不需培养mycoplasma作为正反应对照组,避免可能之污染。 缺点︰PCR反应很灵敏,易有伪阳性结果。此方法尚在评估中,故结果仅作为参考和内部品管之一部份。 材料与设备: ATCC mycoplasma detection kit:可作50~100 reactions. 提供1st stage primer mixture与2nd stage primer mixture positive control DNA (A. laidlawii
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