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- 4327179
- 2025年07月14日
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货号：4327179
产品名称：FG,MGB-NFQ TAQMAN PROBE (FOR SET)
品牌：abi
关键字：abi，Applied Biosystems公司，4327179，FG,MGB-NFQ TAQMAN PROBE (FOR SET)
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文献和实验TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
Real-Time PCR: the TaqMan Method
utilizes a system that is fairly easy to grasp conceptually. First, we must take a look at the TaqMan® probe. Figure 1. The Taqman probe. The red circle represents the quenching dye that disrupts the observable signal from the reporter dye
fluorescence is quantitative for the amount of PCR product produced The Service uses a nonfluorescent quencher dye (NFQ), which raises the signal –to –noise ratio, linked to the minor groove binder (MGB), which allows to make
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