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- 保存条件：
2-8°C
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
10mg/ml
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文献和实验线粒体荧光探针大全：TMRM，Mitotracker，JC-1
by active mitochondria and well retained during cell fixation.16 Because the MitoTracker Orange, MitoTracker Red and MitoTracker Deep Red probes are also retained following permeabilization, the sample retains the fluorescent staining pattern characteristic
How to perform automated counts of fluorescently stained cells.
propidium iodide / mL PBS) Hemocytometer U.V. fluorescent microscope with camera Computer with ImageJ and image cropping software. Procedure Fix Cells by diluting them 50% in EtOH. 1. To an eppendorf add 0.1 mL cells in PBS or media 2.
3. Pipette the top 1 ml of supernatant to a fresh 1.5 ml microcentrifuge tube containing 0.2 ml 20% PEG/2.5 M NaCl to precipitate the phage particles. Mix by inverting several times and incubate for 15-30 minutes at room temperature. 4. Centrifuge for 15
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