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文献和实验Preparing Colloidal Gold for Electron Microscopy
Warm up solutions A and B to 60°C and mix them while stirring. When the red color has formed heat up to 95°C and cool the solution on ice. The larger particles (where lower concentrations of tannic acid are used) take longer to form and the red
Preparing Colloidal Gold for Electron Microscopy
up solutions A and B to 60°C and mix them while stirring. When the red color has formed heat up to 95°C and cool the solution on ice. The larger particles (where lower concentrations of tannic acid are used) take longer to form and the red color
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
Filter with Whatman No.1 paper to remove particles; store at 4°C. 0.1% Triton X-100 in 0.1% Sodium Citrate (SSC) Mix: 2ml 20x SSC 400µl Triton X-100 398ml H2O 100mM Tris-Hcl, pH 8.2
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