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文献和实验Use of Immunogold with Silver Enhancement
Although gold particles are readily detectable by transmission electron microscopy, they can be difficult to visualize by bright-field light microscopy. If the particle size is large enough and the labeling dense enough, the gold particles
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
with a suitable fluorescent marker in the target neurons. Image axons at a similar magnification (0.2 um/pixel or higher magnification) and position under the crosshairs (fig.7). 7) Open the laser safety shutter and trigger the ablation laser
, a modification of this method employing diatomaceous earth (19-22) sometimes is used for isolation of double-stranded templates for DNA sequencing with fluorescent primers. After removal of the precipitated proteins and membranes, the plasmid containing
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