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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
2-(1-Aminoethyl)-N,N-dimethylaniline dihydrochloride
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
1909336-50-0
- 规格:
10mg///100mg///200mg
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文献和实验for each d12 mm coverslip, 25 µl for square coverslip and 50µl for full size coverslip• Seal the coverslip with nail polish• Store in 4 C up to 2 weeksDAPI stock• 4’, 6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) from Sigma (Cat# D-9542), 1 mg 1mg
200,000 dpm samples in 300 µl IMP buffer. 4) To preclear samples, add 50 µl of control (normal rabbit serum, mouse isotype control, etc.) to each tube and 25 µl of Protein G slurry (shake to suspend slurry before pipetting) and incubate for 1 h at 4 C
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
min after the seeding and then every 1 h for a period of 96 h. Recombinant TGF-β1 (Millipore) treatment with various concentrations in triplicate was performed 24 h after the seeding of the cells. Formation of contractile microfilaments was blocked
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