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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
D17-4-Amino-2,2,6,6-tetramethylpiperidine-1-oxyl
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:70588-04-4;unlabelled:14691-88-4
- 规格:
10mg///100mg///200mg
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文献和实验Southern Blotting: DNA Transfer
' (small) to 30' (large). 4. Cut nylon membrane (MSI 0.45 micron #N04HY00010) and several pieces of blotting (e.g. Schleicher and Schuell GB002) paper to the same size as the gel. Wet the nylon with dH20, then soak in 5x SSC. 5. Assemble sandwich
Southern Blotting(Fred Hutchinson Cancer Research Cente
). 3. Neutralization: Wash in (0.5 M Tris pH 7.0, 3.0 M NaCl) for 20' (small) to 30' (large). 4. Cut nylon membrane (MSI 0.45 micron #N04HY00010) and several pieces of blotting (e.g. Schleicher and Schuell GB002) paper to the same size as the gel. Wet the nylon
Southern Blotting:DNA Transfer
) 2. Denaturation: Wash gel in (0.5 M NaOH, 1.5 M NaCl) for 20' (small) to 30' (large). 3. Neutralization: Wash in (0.5 M Tris pH 7.0, 3.0 M NaCl) for 20' (small) to 30' (large). 4. Cut nylon membrane (MSI 0.45 micron #N04HY00010
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