- ￥639 - 1816
- 亚科因(Abbkine)
- 湖北武汉
- KTB1118
- 2025年11月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃避光保存，6个月
- 保质期：
4℃避光保存，6个月
- 英文名：
CheKine™ Micro Lactate Dehydrogenase (LDH) Assay Kit（Dinitrobenzene Hydrazine Assay）
- 库存：
999
- 供应商：
亚科因(Abbkine)
- 规格：
96 T/96 S/96T x5 / 480S 1816
| 规格： | 96 T/96 S | 产品价格： | ￥639.0 |
|---|---|---|---|
| 规格： | 96T x5 / 480S 1816 | 产品价格： | ￥1816.0 |
CheKine™ 乳酸脱氢酶（LDH）活性检测试剂盒（二硝`基`苯`肼比色法）
CheKine™ Lactate Dehydrogenase (LDH) Assay Kit（Dinitrobenzene Hydrazine Assay）
产品核心参数
- 货号：KTB1118
- 检测指标：乳酸脱氢酶（LDH）活性定量
- 检测方法：比色法（二硝`基`苯`肼法）
- 样本兼容性：动植物组织、细胞裂解液、细菌、血清（浆）及细胞培养液
- 灵敏度：31.25 nmol/mL
- 检测范围：31.25 - 2000 nmol/mL
试剂盒组分
| 组分 | 保存要求 |
|---|---|
| • Extraction Buffer | 4℃避光 |
| • Reagent I | |
| • Reagent II | |
| • Reagent III | |
| • Reagent Ⅳ | |
| • Reagent Ⅴ | |
| • Standard |
实验关键点
- 需自行建立标准曲线进行定量计算
- 强烈建议样本均一化处理（推荐BCA法蛋白定量）
- 操作需在通风橱内佩戴防护装备（生化试剂具刺激性/毒性）
- 首次实验请进行预实验优化条件
技术背景
乳酸脱氢酶（LDH）催化丙`酮酸与乳酸的可逆转化反应，伴随NAD⁺/NADH互变，是糖酵解途径关键酶。广泛存在于细胞质中，其活性变化可反映细胞代谢状态及膜完整性。
规格与交付
| 规格 | 价格(￥) | 货期 | 运输条件 |
|---|---|---|---|
| 96 T/96 S | 639 | 现货（次日发货） | 蓝冰冰袋运输 |
| 96T x5 / 480S | 1816 | 现货（次日发货） |
保存与安全
- 保存期限：4℃避光保存6个月
- 警示：组分含刺激性/毒性物质，请严格遵循实验室安全规范
检索关键词：乳酸脱氢酶，LDH，细胞代谢检测，糖酵解分析
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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
CheKine™ Lactate Dehydrogenase (LDH) Activity Assay Kit (Dinitrophenylhydrazine Colorimetric Method)
CheKine™ Lactate Dehydrogenase (LDH) Assay Kit (Dinitrobenzene Hydrazine Assay)
Key Product Parameters
- Catalog Number: KTB1118
- Detection Target: Quantitative measurement of lactate dehydrogenase (LDH) activity
- Assay Method: Colorimetric method (Dinitrophenylhydrazine method)
- Sample Compatibility: Animal/plant tissues, cell lysates, bacteria, serum (plasma), and cell culture media
- Sensitivity: 31.25 nmol/mL
- Detection Range: 31.25 - 2000 nmol/mL
Kit Components
| Component | Storage Requirements |
|---|---|
| • Extraction Buffer | 4°C protected from light |
| • Reagent I | |
| • Reagent II | |
| • Reagent III | |
| • Reagent Ⅳ | |
| • Reagent Ⅴ | |
| • Standard |
Critical Experimental Notes
- Establish standard curves for quantitative calculations
- Strongly recommend sample normalization (BCA protein quantification recommended)
- Perform operations in fume hood with protective equipment (biochemical reagents are irritant/toxic)
- Conduct preliminary experiments for condition optimization during first use
Technical Background
Lactate dehydrogenase (LDH) catalyzes the reversible conversion between pyruvate and lactate, accompanied by NAD⁺/NADH interconversion, serving as a key enzyme in glycolysis. Widely present in cytoplasm, its activity changes reflect cellular metabolic status and membrane integrity.
Specifications & Delivery
| Specification | Price (¥) | Lead Time | Shipping Conditions |
|---|---|---|---|
| 96 T/96 S | 639 | In stock (shipped next day) | Blue ice packs |
| 96T x5 / 480S | 1816 | In stock (shipped next day) |
Storage & Safety
- Shelf Life: 6 months at 4°C protected from light
- Warning: Contains irritant/toxic components; strictly follow laboratory safety protocols
Search Keywords: Lactate dehydrogenase, LDH, Cell metabolism assay, Glycolysis analysis
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Note: The above information may change due to product optimization and upgrades. For specific details, please consult with AcroBio Company.
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文献和实验Lactate Dehydrogenase (LDH) Assay Kit（Dinitrobenzene Hydrazine Assay）
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