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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1)
- 抗体英文名:
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1)
- 供应商:
上海善然生物科技有限公司
- 保存条件:
2-8℃
- 规格:
100ug
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody
RB6-8C5
Catalog# / Size
108422 / 100 µg
Clone
Regulatory Status RUO
Other Names Gr-1
Isotype Rat IgG2b, κ
Description Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a
glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and
macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with
granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow
cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of
antitumor immunity.
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文献和实验,一组使用 NaHS 处理,一组则不作处理;然后用荧光马来酰亚胺染料 Alexa Fluor 488 C5 maleimide(下文简称MAL,Invitrogen,A10254)标记半胱氨酸上的硫醇基团,使正常的半胱氨酸通过 -S- 与 MAL 连接,而硫巯基化的半胱氨酸通过二硫键 -S-S- 与 MAL 连接,如下图: 图 1. 文献 Fig. 6G,硫巯基化分析步骤 接下来加入 DTT,硫巯基化蛋白上特有的二硫键就会被解开,导致荧光 MAL 分子脱落,荧光信号减弱。所以,荧光信号发生变化就验证
-mouse CD3e (CD3 e chain); 2) Biotin-anti-mouse CD11b; 3) Biotin-anti-mouse CD45R/B220; 4) Biotin-anti-mouse Ly-6G and Ly-6C (Gr-1); 5) Biotin-anti-mouse TER-119/Erythroid Cells (Ly-76) 磁分离细胞的重要指标是:
')2 Fragment(Alexa Fluor® 555 Conjugate)#4413 Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 647 Conjugate)#4414 抗小鼠: Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4408 Anti-Mouse IgG(H+L), F(ab')2 Fragment(Alexa Fluor
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