- ￥4774
- BioLegend
- 124210
- 美国
- 2025年07月14日
- 免疫荧光，流式
- Armenian Hamster
- Mouse, Rat, Human
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- 详细信息
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- 技术资料
- 抗体名：
PE anti-mouse/rat/human CD27
- 抗体英文名：
PE anti-mouse/rat/human CD27
- 浓度：
0.2 mg/ml
- 应用范围：
免疫荧光，流式
- 宿主：
Armenian Hamster
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
Armenian Hamster
- 保质期：
2-3年左右
- 适应物种：
Mouse, Rat, Human
- 标记物：
PE
- 克隆性：
单克隆
- 保存条件：
2-8度
- 形态：
液体
- 规格：
200ug
PE anti-mouse/rat/human CD27 Antibody
LG.3A10
Catalog# / Size 124209 / 50 µg
124210 / 200 µg
Clone
Regulatory Status RUO
Other Names T14, S152, Tp55, TNFRSF7
Isotype Armenian Hamster IgG
Description CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR)
superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and
thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell
interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to
effector cells.
Product Details
Verified Reactivity Mouse, Rat, Human
Antibody Type Monoclonal
Host Species Armenian Hamster
Immunogen Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation The antibody was purified by affinity chromatography, and conjugated with PE under optimal
conditions.
Concentration 0.2 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged
exposure to light. Do not freeze
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Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
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