- ￥2235.10
- BioLegend
- 103902
- 美国
- 2025年08月18日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Purified anti-mouse/rat CD49e
- 抗体英文名：
Purified anti-mouse/rat CD49e
- 供应商：
上海善然生物科技有限公司
- 保存条件：
2-8℃
- 规格：
500ug
Purified anti-mouse/rat CD49e Antibody
HMα5-1
Catalog# / Size 103902 / 500 µg
Clone
Regulatory Status RUO
Other Names α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Isotype Armenian Hamster IgG
Description CD49e is a 135 kD protein, also known as α integrin or VLA-5 α chain. It is a member of the
integrin family, expressed on thymocytes, pro-B cells, activated T cells, and mast cells. CD49e
associates with CD29 (integrin β chain) to form the fibronectin receptor (VLA-5). CD49e plays
a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29
(VLA-5) is fibronectin. The HMα5-1 antibody has been shown to partially block CD49e
mediated interactions. It has also been observed that plate-bound HMα5-1 antibody enhances
IgE mediated degranulation and that in vivo injection of HMα5-1 antibody, in combination with
anti-CD49d and anti-CD61 antibodies, blocks passive cutaneous anaphylaxis.
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
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