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- BioLegend
- 119307
- 美国
- 2025年08月15日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
PE anti-mouse CD34
- 抗体英文名：
PE anti-mouse CD34
- 供应商：
上海善然生物科技有限公司
- 保存条件：
2-8℃
- 规格：
50ug
PE anti-mouse CD34 Antibody
MEC14.7
Catalog# / Size 119307 / 50 µg
Clone
Regulatory Status RUO
Other Names Mucosialin
Isotype Rat IgG2a, κ
Description CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have
been reported to be generated by alternative splicing. This antigen is expressed on
hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to
function as an adhesion molecule for early hematopoietic progenitors mediating the
attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on
serine residues by PKC.
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
与在各种检测方案中所应用的不同技术。并就外周血干细胞计数标准方案达成了一致。推荐方案中要求标本需预先作白细胞计数，用来染色的标本体积根据对 CD34+ 细胞数量估算来决定，分析时要求收集足够的有统计学意义的标本数量。方案中推荐使用双标记方法染色，其中包括将 2 ul 的 PE 标记的 CD3 抗体和 10ul 的 FITC 标记的 CD34 抗体（ QBEnd10 ）加入 250ul 的全血进行染色，室温下孵育 20 分钟，每 5 分钟用移液器吹打混匀一次；随后加入 Ortho 公司的红细胞裂解液孵育
RNAi-Inducing Lentiviral Vectors for Anti-HIV-1 Gene Therapy
RNA interference (RNAi)-based gene therapy for the treatment of HIV-1 infection provides a novel antiviral approach. For delivery of RNAi inducers to CD4+ T cells or CD34+ blood stem cells, lentiviral vectors are attractive
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