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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
CIS-13-DOCOSENOL
- 库存:
99
- 供应商:
联硕生物
- CAS号:
629-98-1
- 规格:
250mg
纯度:≥95%
保存温度:RT
英文名称:CIS-13-DOCOSENOL
分子式:C22H44O
XUQVMZKOTENHYIAJBLRDWPCGFS
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文献和实验Protein Expression and Purification of RPA70-AB (181-422)
with Buffer A and collect flow through and two washes of approx two column size.17.The final protein yields vary from 10-15 mg/L.Note:To prepare 15N or13℃15N labeled samples follow the same procedure except that in steps 2 and 3 M9 minimal medium
Yeast Chromatin Immunoprecipitation (ChIP)
ice, and store at �70 degrees C Day 5 1. Thaw extracts on ice and transfer a volume of extract (1mg total protein) to a new 1.5 ml tube for IP 2. Include necessary controls (i.e. no Ab control; untagged strain + Ab) 3. Bring all 1mg aliquots
Yeast Chromatin Immunoprecipitation (ChIP)
total protein)to a new 1.5 ml tube for IP 2.Include necessary controls (i.e.no Ab control; untagged strain+ Ab) 3.Bring all 1mg aliquots to 200μl with ChIP lysis buffer+protease inhibitors,mix well (thorough mixing is very important) 4.Transfer 2μl
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