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文献和实验A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation
The thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) has been widely used to make sister chromatid differentiation (SCD) evident in metaphase chromosomes of cells grown for two cycles in BrdU and, thus, containing varying
, or other biologically active molecules. In this chapter, we present a technique, which allows identification of specific DNA sequences by fluorescence in situ hybridization (FISH) and simultaneously analyses the incorporation of a thymidine analogue, 5-bromo
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Acad. Sci. USA 85 :6342. Holt, S.J., and D.G. O'Sullivan. 1958. Studies in enzyme cytochemistry I. Principles of cytochemical staining methods. Proc. Royal Soc. (London) 148B :465. Holt, S.J., and P.W. Sadler. 1958. Studies in enzyme
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