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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
26-DEOXYCIMICIFUGOSIDE
- 库存:
99
- 供应商:
联硕生物
- CAS号:
214146-75-5
- 规格:
5mg
纯度:HPLC≥98%
保存温度:2-8℃
英文名称:26-DEOXYCIMICIFUGOSIDE
分子式:C37H54O10
MQTKCPABNIVXOHRLGSEFWJDYZU
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
26- counterstain with rabbit anti-goat-AP 1:200 27- wash thrice 28- develop AP (see protocol) HRP development protocol (AEC): Developing solution: For 50 ml developing solution add in order: Aminoethylcarbazole (20 mg tablets
Spin down in Sorvall--5 K, 5 minutes, 4°C. Drain. Resuspend cells in 5 mL HEPES/sorb to wash. Spin down (5 K, 5 minutes, 4°C). Drain. Resuspend in 5 mL HEPES/sorb with 0.5 mM PMSF (add all inhibitors immediately before use). Add 60 µL 1
Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
dilution buffer). Sample volumes should be between 200 and 600 uls. Add 1 ug of antibody to each Ab sample (this may vary for individual antibodies). 13. Incubate on a rotating platform at 4 0 C overnight (or for at least 3 hours
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