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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
2,3,4,6-TETRA-O-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE
- 库存:
99
- 供应商:
鹿森生物
- CAS号:
51642-81-0
- 规格:
100mg
纯度:97%
保存温度:2-8℃
英文名称:2,3,4,6-TETRA-O-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE
分子式:C14H21NO9
ADEIHJMYCXKROPLBQFSGUTZWVN
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文献和实验Sucrose Density Gradient Fractionation
of O.D.=1 culture into 100 ml total YPD)If appropriate,after 1 hr add 2.5 uM alpha factor (421μl of stock (1 mg/ml)to 100 ml of culture)(we want it present during the last hour of growth)Wait 1 doubling period (about 2 hours)until O.D.600 equals
Sucrose Density Gradient Fractionation of Yeast Membranes
Resuspend in 100 ml pre-warmed YPD so that O.D.600 = 0.5 (50 ml of O.D.=1 culture into 100 ml total YPD) If appropriate, after 1 hr add 2.5 uM alpha factor (421 ul of stock (1 mg/ml) to 100 ml of culture) (we want it present during the last hour
, after 1 hr add 2.5 uM alpha factor (421 ul of stock (1 mg/ml) to 100 ml of culture) (we want it present during the last hour of growth) Wait 1 doubling period (about 2 hours) until O.D.600 equals about 1 Add 1 ml of COLD 1M NaN3 to 100 ml
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