- ￥389.88
- AMEKO
- 中国
- AB64509-125ml
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 库存：
99
- 供应商：
鹿森生物
- 规格：
125ml
保存温度：RT
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文献和实验Yeast Chromatin Immunoprecipitation (ChIP)
which can then be discarded) 7. Emulsify the insoluble pellets with their supernatants and transfer to 2.0 ml round bottom tubes (the chromatin is primarily in the insoluble fraction) 8. Sonicate extracts in Breeden Lab water bath sonicator at level 5, 100% duty, 4 x 30
Yeast Chromatin Immunoprecipitation (ChIP)
with their supernatants and transfer to 2.0 ml round bottom tubes (the chromatin is primarily in the insoluble fraction)8.Sonicate extracts in Breeden Lab water bath sonicator at level 5,100% duty,4 x 30 sec with 30 sec pauses on ice between runs (sonicator output
( I make this fresh) (final conc. 0.1 mg/ml) 1.0 µl beta-mercaptoethanol - Incubate in a 50 to 60° water bath; gently resuspend by inversion at 1'', 3'', 10'' to achieve a ~uniform suspension instead of one big glop floating in liquid.
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