- ￥480 - 1600
- Solarbio|ActivAb
- 北京
- K112663P
- 2025年08月13日
- WB;IHC
- Rabbit
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein of human RNF25
- 亚型：
IgG
- 保存条件：
Store at -20℃. Avoid freeze / thaw cycles.
- 克隆性：
Polyclonal Antibody
- 标记物：
Biotin，Cy3，Cy3.5，Cy5，Cy5.5，Cy7，FITC，HRP，RBITC，Solar Fluor 350，Solar Fluor 405，Solar Fluor 488，Solar Fluor 532，Solar Fluor 555，Solar Fluor 568，Solar Fluor 594，Solar Fluor 633，Solar Fluor 640，Solar Fluor 647，Solar Fluor 660，Solar Fluor 680，Solar Fluor 750
- 适应物种：
Human;Mouse
- 抗原来源：
Recombinant protein of human RNF25
- 目录编号：
64320
- 级别：
实验级别
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB;IHC
- 浓度：
WB 1:2000-5000. IHC 1:25-100.
- 靶点：
RNF25
- 抗体英文名：
Anti-RNF25 Polyclonal Antibody
- 抗体名：
环指蛋白25抗体
- 规格：
20ul/100ul/50ul
| 规格： | 20ul | 产品价格： | ￥480.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1600.0 |
| 规格： | 50ul | 产品价格： | ￥960.0 |
| 基本信息 | |
| 英文名称 | Anti-RNF25 Polyclonal Antibody |
| 中文名称 | 环指蛋白25抗体 |
| 宿主 | Rabbit |
| 克隆类型 | Polyclonal Antibody |
| 亚型 | IgG |
| 交叉反应 | Human;Mouse |
| 预测交叉反应 | Bovine |
| 应用 | WB;IHC |
| 稀释比例 | WB 1:2000-5000. IHC 1:25-100. |
| 密度 | |
| 靶标 | RNF25 |
| 别名 | AO7;EC 6.3.2.-;FLJ13906;ring finger protein 25E3 ubiquitin-protein ligase RNF25;ring finger protein AO7 |
| 背景说明 | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF25 (RING finger protein 25), also known as AO7, is a 459 amino acid protein that contains one RWD domain and one RING-type zinc finger. Via its RING-type zinc finger (a common domain that contains a conserved cysteine-rich region and is found in a number of viral and eukaryotic proteins), RNF25 is thought to exhibit E2-dependent E3 ubiquitin-protein ligase activity. Specifically, the RING finger of RNF25 interacts with an E2 ligase and, through this interaction, facilitates the ubiquitination of target proteins. RNF25 may also regulate the transcriptional activity of NF-kappa-B, a protein that plays important roles in cell survival, inflammation response, host defense and proliferation. |
| Swiss Prot | Q96BH1 |
| Gene ID | 64320 |
| 亚细胞定位 | Cytoplasm Nucleus |
| 免疫原 | Recombinant protein of human RNF25 |
| Public Immunogen Range | 1-350aa |
| 理论分子量 | 51kDa |
| 实际分子量 | 64kDa |
| EC | |
| 纯化方法 | Affinity purification |
| 储存液 | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
a simple ELISA method to detect human anti-antibody response.
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