Anti-RNF25 Polyclonal Antibody

产品简介

中文名称	环指蛋白25抗体
靶标	RNF25
别名	AO7;EC 6.3.2.-;FLJ13906;ring finger protein 25E3 ubiquitin-protein ligase RNF25;ring finger protein AO7
背景资料	Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF25 (RING finger protein 25), also known as AO7, is a 459 amino acid protein that contains one RWD domain and one RING-type zinc finger. Via its RING-type zinc finger (a common domain that contains a conserved cysteine-rich region and is found in a number of viral and eukaryotic proteins), RNF25 is thought to exhibit E2-dependent E3 ubiquitin-protein ligase activity. Specifically, the RING finger of RNF25 interacts with an E2 ligase and, through this interaction, facilitates the ubiquitination of target proteins. RNF25 may also regulate the transcriptional activity of NF-kappa-B, a protein that plays important roles in cell survival, inflammation response, host defense and proliferation.
Swiss Prot	Q96BH1
亚细胞定位	Cytoplasm Nucleus
宿主	Rabbit
克隆类型	Polyclonal Antibody
亚型	IgG
预测交叉反应	Bovine
蛋白分子量	51kDa
实际分子量	64kDa
稀释比例	WB 1:2000-5000. IHC 1:25-100.
免疫原	Recombinant protein of human RNF25
Public Immunogen Range	1-350aa
纯化方法	Affinity purification
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
保存	Store at -20℃. Avoid freeze / thaw cycles.
交叉反应	Human;Mouse
应用	WB;IHC
规格	50ul 100ul
单位	支

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

"Western blot analysis with RNF25 antibody diluted at 1:3000;Lane: 293T

Immunohistochemistry of paraffin-embedded human colorectal cancer with RNF25 antibody diluted at 1:50.

Immunohistochemistry of paraffin-embedded human pancreatic cancer with RNF25 antibody diluted at 1:50.

Immunohistochemistry of paraffin-embedded human testis cancer with RNF25 antibody diluted at 1:50.