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- 2025年08月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pUC19-PJ23119-OmpA-mCherry-dps(Rhizobium)
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pUC19-PJ23119-OmpA-mCherry-dps(Rhizobium) |
| 别称 | DQ078256.1 |
| 基因长 | 1077bp |
| 质粒宿主 | 大肠杆菌 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 根瘤菌 |
| 原核抗性 | Amp |
| 荧光标记 | 红色 |
| 启动子 | J23119 |
| 复制子 | pUC |
| 感受态 | DH5a |
| 温度 | 37度 |
| 背景载体 | pUC19-PJ23119-OmpA-mCherry |
| 正向引物 | MCHERRY-F |
| pT7-6×His-PNPLA5(human)-6×His |
| pT7-6×His-RAB11A(human)-6×His |
| pT7-6×His-SacB |
| pET-GST-NLRP3(human) |
| pT7-6×His-STAT2(human) |
| pT7-6×His-STAT1(human) |
| pT7-Mito-henAsCas12a-HF1(E174R-N282A-S542R-K548R)-6×His(AAS1935) |
| pT7-6×His-HDAC10(human)-6×His |
| pT7-6×His-GFP-FOSL1(human)-6×His |
| pT7-6×His-Stag-Pkm(mouse)-6×His |
| pT7-6×His-Stag-Lonp2(mouse)-6×His |
| pT7-6×His-AKT2(human) |
| pT7-6×His-FGF21(cattle)-6×His |
| pT7-6×His-Arih1(mouse) |
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文献和实验DNA Isolation from Rhizobium by Phenol Chloroform Method
Grow Rhizobium cells in 5 ml of YEMB at 200 rpm till the O.D (600 nm) reaches 0.6-0.8. Pellet the cells by centrifugation at 10,000 rpm for 15 mins. Wash the pellet with TE buffer (10T/1E). Dissolve the pellet in 400 μ
Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes
. It was originally designed to isolate unique DNA sequences from Rhizobium spp. (6 ), but is applicable to any group of bacteria to generate species- or strain-specific DNA probes.
Abstract: It is very difficult to isolate Rhizobium DNA due to the gum production by the organism. Hence we have designed a protocol for efficient isolation of DNA from the organism for further gene amplification.Procedure 1.Grow Rhizobium cells
技术资料