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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
pUT-mini-Tn5-CmR
- 保质期:
3个月~12个月
- 供应商:
上海禾午生物有限公司
- 保存条件:
负20℃&负80℃
- 规格:
干粉&甘油菌
| 名称 | pUT-mini-Tn5-CmR |
| 别称 | - |
| 质粒宿主 | 大肠杆菌 |
| 质粒用途 | 基因编辑 |
| 片段类型 | ORF |
| 片段物种 | 空载体 |
| 原核抗性 | Amp |
| 筛选标记 | Chl |
| 启动子 | T7 |
| 复制子 | R6K γ |
| 感受态 | S17-1λpir |
| 温度 | 30度 |
| 背景载体 | pUT-mini-Tn5-Gm |
| 拷贝数 | 低 |




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文献和实验Here, we document a technique to reduce the size of the genome of Pseudomonas putida by using a combinatorial mini-Tn5 -targeted Flp-FRT recombination system. This method combines random insertions with the site-specific Flp-FRT recombination
Single Primer ("Semi-Random") P
GAAACGCTTTCGCGTTTTTCGTGCG Pn: F20 (TP79) Exactly at end. GTTTTTCGTGCGCCGCTTC 4. Tn5-derived chloramphenicol resistance cassette (found in Mud-cam and Tn10d-cam) Po: CMR2 (TP699) 158 bp prior to gene facing upstream. CTTCCCGGTATCAACAGGGACA Pn: CMR
In Vitro Mutagenesis of Brucella Species
Three major techniques have been employed for broad-range in vitro mutagenesis of Brucella species. Shotgun approaches capable of generating large libraries of randomly inserted transposon mutants include Tn5, mariner (Himar1), and mini-Tn5
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