- ￥2052
- AMEKO
- 中国
- AB62696-5mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 库存：
99
- 供应商：
鹿森生物
- CAS号：
1418130-80-9
- 规格：
5mg
纯度：98%
保存温度：2-8℃
分子式：C20H30O5
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
endogenous peroxidase by incubating in TBS-T 0.1%NaN 3 and 0.3% H 2 O 2 for 30 min. Wash thrice. Top of Page Choose next steps: HRP-conjugated secondary Ab Tyramide amplification Biotin-conjugated secondary Ab
RNA in situ protocol for DNA and RNA probes
for 3-5 min. with 50 ug/ml proteinase K in PBT**. **When using riboprobes, it is necessary to reduce or eliminate the proteinase K treatment. We use 4 ug/ml proteinase K for 3 min. 11) Stop the protease by washing 2x with PBT containing 2 mg/ml
RNA in situ protocol for DNA and RNA probes
are carried out at 55oC. 15) Add17 ng (17 ul) of heat-denatured probe to each tube containing 20-50 ul of embryos and 100 ul of hybridization buffer. Hybridize at 48oC (55oC for riboprobes) for 24-48 hrs. 16) Wash the embryos 4-5x in 1 ml of 48oC
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