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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
PJQ-200SK-Rif
- 保质期:
3个月~12个月
- 供应商:
上海禾午生物有限公司
- 保存条件:
负20℃&负80℃
- 规格:
干粉&甘油菌
| 名称 | PJQ-200SK-Rif |
| 别称 | - |
| 质粒宿主 | 大肠杆菌 |
| 质粒用途 | 基因编辑 |
| 片段类型 | ORF |
| 片段物种 | 空载体 |
| 原核抗性 | Rif |
| 筛选标记 | SacB |
| 复制子 | p15A |
| 感受态 | DH5a |
| 温度 | 37度 |
| 背景载体 | pJQ200SK |




| pT7-6×His-PKM(human)-6×His |
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| PJQ-200SK-Rif |
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| pT7-6×His-THAP11(human-opt-ecoli)-EGFP |
| pT7-TrxA-6×His-STAT6(human)(113-658aa) |
| pTac-GST-Cc2d1a(mouse) |
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文献和实验Agrobacterium growth and transformation (农杆菌培养及转化)
tube, incubate with shaking (200rpm) overnight. Aliquot and freeze remainder of cells, storing at -80°C, to be used with reasonable transformation efficiency at a later date. DAY4 Pour plates TYNG with selection (Rif plus antibiotics
Agrobacterium growth and transformation
tube, incubate with shaking (200rpm) overnight. Aliquot and freeze remainder of cells, storing at -80°C, to be used with reasonable transformation efficiency at a later date. DAY4 Pour plates TYNG with selection (Rif plus antibiotics
Sucrose Density Gradient Fractionation
about 1Add 1 ml of COLD 1M NaN3 to 100 ml culture (we want 10 mM total conc.)Spin down 3 x109 cells (about 100 O.D.equivalents (100 ml of OD = 1): 2750 X g.,10 min.,30℃ in lab clinical centrifugeWash 1x with 10 ml SK buffer in 15 ml falcon tubes (see recipe
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