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文献和实验Coupling Antibodies to Protein A or G
1. use 2 mg of antibody per ml wet beads (use appropriate antibody/protein A or G combination). 2. mix antibodies with beads and bind at room temperature for at least 1 hr (on roller). 3. wash the beads twice with 10 volumes borate buffer, spin
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
for 5 min. Transfer 600 μL the supernatant into a new 2 ml tube and add 200 μL Buffer SP2. Mix the sample throughly by vortexing. 5. Incubate on ice for 5 minutes. Centrifuge at 13,000 x g in a microcentrifuge for 5 minutes. 6. Carefully
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