Immobilon®-E PVDF转印膜预切片膜, 0.45

Western实验步骤

培养液、蛋白酶抑制剂等）及其他干扰物质；b. 样品浓度应适中，1×SDS凝胶加样缓冲液建议用量：贴壁细胞按10cm2培养皿/0.1ml,悬浮细胞按5×106细胞/0.1ml比例加入；c. SDS对蛋白质变性和电泳分离至关重要，浓度应控制在2-10%之间，并根据具体需要调整；d. 制备好的样品可以在-20℃保存，但时间不宜过长，并避免反复冻融，否则蛋白会发生降解；e. 内参对实验结果至关重要，应选择合适的内参。二、电泳电泳分为非变形凝胶电泳和变性凝胶电泳，Western Blotting中常用的是不连续缓冲

Western Blotting Protocols

  Required Equipment Shallow trays, large enough to hold blot Glass plates Plastic wrap (e.g., Saran™ film), freezer bag, or sheet protector Autoradiography film and cassette Dark room Autoradiography film

Endothelial Cell Systems - Instructions For Use

the flask. For each 25 cm2 of cells to be subcultured, allow 3 ml of Clonetics® Trypsin/EDTA (T/E) to thaw and come to room temperature. For each 25 cm2 of cells to be subcultured, allow 5 ml of Clonetics® HEPES Buffered Saline Solution (HEPES-BSS