Immobilon®-P PVDF转印膜，0.45um孔径，

IF=20.3！外泌体热点之牙周组织再生研究

近期，来自广州海洋实验室的陈凯教授，昆明医科大学口腔医学院的杨禾丰教授和胡江天教授团队在 Bioactive Materials 杂志发表了题为「miR-423-5p-enriched small extracellular vesicles drive periodontal regeneration via Sfrp2+ cell expansion」的科研论文，IF = 20.3。   研究背景： 牙周缺损（periodontal defect）是牙周炎引发的成年人牙齿脱落的主要原因

Reverse Transcription of RNA- 20l rxn

for 10mins. Notes All reagents should be diluted in dH20 that has been dep''d and autoclaved. Add all reagents except the RT and RNA and let them sit in the presence of the RNase for 15 mins. I use 1.5 ug of Poly A+ RNA in this rxn

A protocol for cleaning and reusing the large 25 x 25 cm plates

We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV