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- Millipore
- IPVH20200
- 2025年08月12日
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文献和实验Reverse Transcription of RNA- 20l rxn
for 10mins. Notes All reagents should be diluted in dH20 that has been dep''d and autoclaved. Add all reagents except the RT and RNA and let them sit in the presence of the RNase for 15 mins. I use 1.5 ug of Poly A+ RNA in this rxn
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
Clonality - X Chromosome Inactivation Assay
) 10X TBE (1 M Tris, 0.9 M Boric Acid, and 0.01 M EDTA) (Life Technologies) Loading dye (95% formamide, 20 mM EDTA, 0.05% bromophenol blue, 0.05% xylene cyanol) 2. Methods TIP: Investigators must be especially careful when using this methodology
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