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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
805-005-180
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
AffiniPure® Bovine Anti-Goat IgG (H+L) (min X Bov,Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
- 规格:
1mg
产品简介:
品牌:Jacksonimmuno
货号:805-005-180
产品名称:AffiniPure® Bovine Anti-Goat IgG (H+L) (min X Bov,Ck,GP,Sy Hms,Hrs,Hu,Ms,Rb,Rat Sr Prot)
https://www.jacksonimmuno.com/catalog/products/805-005-180
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文献和实验( min X….Mouse, Rat Sr Prot )。 以下是圆括号中所使用的一些简称: Bov = Bovine Ck = Chicken Gt = Goat GP = Guinea Pig Sy Hms = Syrian Hamster Hrs = Horse Hu = Human Ms = Mouse Rb = Rabbit Shp
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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