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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
315-065-020
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Biotin-SP-conjugated AffiniPure® Rabbit Anti-Mouse IgM, µ Chain Specific
- 规格:
1.5ml
产品简介:
品牌:Jacksonimmuno
货号:315-065-020
产品名称:Biotin-SP-conjugated AffiniPure® Rabbit Anti-Mouse IgM, µ Chain Specific
https://www.jacksonimmuno.com/catalog/products/315-065-020
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
volume of Dynabeads taken from the vial (step 2). 2. Activation of Mouse T Cells 1) Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2) Add 2 µl Dynabeads Mouse T-Activator CD3/CD
In situ hybridisation to alpha satellite sequences (chromosome specific)
solution and replace with mouse anti-biotin antibody diluted 1:100 in blocking solution for 40 minutes at 37C. We currently use DAKO antibody #M0743, 100-200m l per slide. (10) Wash with blocking solution for 10 minutes (11) Incubate with rabbit
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