- ￥2403
- Jacksonimmuno
- 美国
- 309-035-011
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 目录编号：
309-035-011
- 库存：
详询
- 供应商：
广州市左克生物
- 浓度：
见包装
- 抗体英文名：
Peroxidase-conjugated AffiniPure® Rabbit Anti-Human Serum IgA, α Chain Specific
- 规格：
1.5ml
产品简介:
品牌：Jacksonimmuno
货号：309-035-011
产品名称：Peroxidase-conjugated AffiniPure® Rabbit Anti-Human Serum IgA, α Chain Specific
https://www.jacksonimmuno.com/catalog/products/309-035-011
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文献和实验ELISA Procedure for Measuring Serum Antibody Titer
. Reagents for Horseradish Peroxidase Detection1. Goat anti-species IgG whole molecule/horseradish peroxidase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
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