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- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
115-195-075
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
6nm Colloidal Gold-AffiniPure® Goat Anti-Mouse IgM, µ Chain Specific (min X Hu,Bov,Hrs Sr Prot) (EM Grade)
- 规格:
0.5ml
产品简介:
品牌:Jacksonimmuno
货号:115-195-075
产品名称:6nm Colloidal Gold-AffiniPure® Goat Anti-Mouse IgM, µ Chain Specific (min X Hu,Bov,Hrs Sr Prot) (EM Grade)
https://www.jacksonimmuno.com/catalog/products/115-195-075
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
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