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- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
115-065-207
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Biotin-SP-conjugated AffiniPure® Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific (min X Hu,Bov,Rb Sr Prot)
- 规格:
0.5ml
产品简介:
品牌:Jacksonimmuno
货号:115-065-207
产品名称:Biotin-SP-conjugated AffiniPure® Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific (min X Hu,Bov,Rb Sr Prot)
https://www.jacksonimmuno.com/catalog/products/115-065-207
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
. A cheap alternative is to exploit the relative lower levels of each IgG isotype in serum and on follicular B cells to stain with isotype-specific, conjugated, anti-mouse secondary antibodies. IgG isotypes account each for 20-25% ot total serum
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining
Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
secretory pathway. The N-terminal cysteine is then conjugated with a biotin thioester to produce a biotinylated, glycosylated IgG1 Fc using native chemical ligation.
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