AffiniPure® Goat Anti-Rat IgG

E L I S A w i t h p l a t e l e t s

for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS

Rat x Rat Hybridomas

. In addition, certain subclasses of rat IgG have been found to interact efficiently with human Fc receptors and the human complement system. More important perhaps in practical terms is that, when grown as an ascites, up to 30 mL of fluid containing 1–5 mg/mL

Jackson ImmunoResearch 二抗选用指南

, 这种抗体在检测亚美尼亚仓鼠IgG 单克隆抗体时，它的效果没有抗亚美尼亚仓鼠IgG 好。 注意：抗亚美尼亚仓鼠IgG(H+L)(min X Bov, Hu, Ms , Rb, Rat Sr Prot) 不能识别所有的亚美尼亚仓鼠单克隆抗体，因为它经相近物种的吸附处理过（粗体字）。因此，使用经过少数物种或少数相近物种的吸附处理过的抗体可达到较好的效果，如抗亚美尼亚仓鼠IgG(H+L)(min X BoV Sr Prot), 除此之外，在小鼠或大鼠免疫球蛋白中也应该检测亚美尼亚仓鼠单抗。