- ￥1768
- Jacksonimmuno
- 美国
- 016-010-084
- 2026年06月25日
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- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 目录编号：
016-010-084
- 库存：
详询
- 供应商：
广州市左克生物
- 浓度：
见包装
- 抗体英文名：
Fluorescein (DTAF)-conjugated Streptavidin
- 规格：
1mg
产品简介:
品牌：Jacksonimmuno
货号：016-010-084
产品名称：Fluorescein (DTAF)-conjugated Streptavidin
https://www.jacksonimmuno.com/catalog/products/016-010-084
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文献和实验Functionality Screen of Streptavidin Mutants by Non-Denaturing SDSPAGE Using Biotin-4-Fluorescein
screening methodologies in mind, it is imperative to detect, prior to the high-density protein production, the bacteria that produce non-functional streptavidin isoforms. Based on the incorporation of biotin-4-fluorescein in streptavidin mutants present
Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
takes place by means of an antibody (or, in the case of biotin, streptavidin) linked to an enzyme, such as alkaline phosphatase or horse-radish peroxidase, which is used to catalyze the detection reaction. A variety of haptens have been employed
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