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- 文献和实验
- 技术资料
- 英文名:
TentaGel S NH2(0.2-0.8 meq/g, 130 µm mesh)
- 库存:
999
- 供应商:
智溯科ZSK
- 规格:
10mg///100mg///200mg
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文献和实验Kamps's Western Blotting Protocol
from the shelf (Margie's).Stain for approximately 20 to 60 minutes.Rinse with water for 1 hr.Dry flat.Be careful.Buffers containing Tween 20 can reduce the binding of antiphosphotyrosine antibodies. Recipes Transfer buffer: Glycine 57.6 g Tris Base 12.0 g
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
selection Lysis buffer: PBS + 1% Triton X-100, supplemented just before use with protease inhibitors (e.g, final concentrations of 2 µg/ml aprotinin , 1 µg/ml leupeptin , and 25 µg/ml phenylmethylsulfonyl fluoride [PMSF]) Phosphate-buffered saline (PBS), ice
E L I S A w i t h p l a t e l e t s
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
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