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- 文献和实验
- 技术资料
- 英文名：
5-Bromo-3-methylbenzobthiophene
- 库存：
999
- 供应商：
智溯科ZSK
- CAS号：
1196-09-4
- 规格：
10mg///100mg///200mg
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文献和实验Southern Blot using Neutral Transfer
volume. Digest overnight,spiking with a bit more enzyme for 2 more hours in the morning. Concentrate your sample by precipitation: adding 10 μl of 3M NaOAc and then 110 μl isopropanol, invert tube and spin 10 min. Remove liquid carefully, let dry 10 min
Sample preparation (preparative gels)
through a mesh with a pore size of 300 µm to separate epithelial cells from stroma and filtered through a nylon mesh with 200 µm pores. After centrifugation at 350 g at 4o C for 10 min, the membranes were permeabilized in 70 % chilled ethanol and the cells
Sample preparation (analytical gels)
membrane with a scalpel. The tissue was then gently pressed through a mesh with a pore size of 300 μm to separate epithelial cells from stroma and filtered through a nylon mesh with 200 μm pores. After centrifugation at 350 g at 4o C for 10 min
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