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- 详细信息
- 文献和实验
- 技术资料
- 库存:
充足
- 英文名:
Add&Read® cAMP Detection Kit
- 供应商:
南京诺唯赞生物科技股份有限公司
- 保存条件:
-30 ~ -15℃储存,运输条件:≤0℃。
- 规格:
1000 tests/5000 tests/20000 tests
产品描述
cAMP Detection Kit可用于检测细胞中cAMP的水平。试剂盒中有一株抗体识别cAMP,标记荧光供体Eu (Anti-cAMP-Eu);cAMP上标记荧光受体A2 (cAMP-A2)。
在溶液中,Anti-cAMP-Eu与cAMP-A2结合,可发生荧光共振能量转移(FRET),使用320 nm的光激发荧光供体Eu,Eu供体发射620 nm的光,此620 nm的光激发荧光受体A2,A2受体发射出665 nm的光。细胞裂解后,细胞内产生的的cAMP被释放到裂解液样本中,会竞争结合Anti-cAMP-Eu,破坏FRET现象。待测细胞中cAMP的浓度与FRET的信号值(665 nm/620 nm的光强度比值)成反比。
产品特点
兼顾窗口和灵敏度:检测窗口大,同时灵敏度高
应用场景广泛:可用于Gs或Gi通路
省时省力:操作步骤简单,节约时间
高通量:搭配自动化设备,实现高通量检测
检测原理

产品组分

储存条件
试剂盒于-30 ~ -15℃保存,≤0℃运输。
▲ cAMP Standard、Anti-cAMP-Eu和cAMP-A2于-30 ~ -15℃保存,首次使用时,请按需分装,避免反复冻融;
▲ Stimulation Buffer和Lysis Detection Buffer于-20 ~ 4℃保存。
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