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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
FM (Fibroblast Medium)
- 规格：
500ml/T
|
货号 |
2301 |
|
产地 |
美国 |
|
缩写 |
FM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
成纤维细胞培养基是为正常人类成纤维细胞体外培养设计的适于其生长的培养基。它是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为HEPES和重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类成纤维细胞体外培养中的增殖和生长，并为其达到理想营养平衡状态提供数量上和质量上的保证。
成纤维细胞培养基包含500 ml基础培养基，10ml胎牛血清(FBS,目录编号0010)，5ml成纤维细胞生长添加物(FGS,目录编号2352)和5 ml青霉素/链霉素溶液(P/S,目录编号0503)
1.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) 'Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer.'
2.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) "Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer." PloS one 9: e96878.
3.) Li F, Weir MD, Chen J, Xu HHK. (2013) "Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity."Dent Mater. 29: 450-61.
4.) Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.
5.) Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.
6.) Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.
7.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) 'TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression.'
8.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) "TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression." PloS one. 8: e77366.
9.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'
10.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) 'B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts.'
11.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.
12.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) "Export of microRNAs and microRNA-protective protein by mammalian cells." Nucleic Acids Res. 38:7248-59.
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文献和实验
1.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) 'Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer.'
2.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) "Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer." PloS one 9: e96878.
3.) Li F, Weir MD, Chen J, Xu HHK. (2013) "Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity."Dent Mater. 29: 450-61.
4.) Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.
5.) Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.
6.) Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.
7.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) 'TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression.'
8.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) "TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression." PloS one. 8: e77366.
9.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'
10.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) 'B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts.'
11.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.
12.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) "Export of microRNAs and microRNA-protective protein by mammalian cells." Nucleic Acids Res. 38:7248-59.
！）。培养皿中可以先涂较薄的一层含血清培养基，再将组织块贴上去，密度高一些，便于细胞融合。4－10小时后加少量培养液，不要让组织块浮起！！以后几天不要动他，最快3－4天就可以有细胞爬出了！ 细胞需要传代时，要先用无菌的小镊子将组织块夹出，再用0.25％胰酶（含0.02％EDTA）消化即可。 如果细胞来自瘢痕疙瘩，在DMEM培养2－3代之后就基本上全是成纤维细胞了，没有特殊原因不需要鉴定。 丁香园网友fanny816che的观点为： 材料:对遗传咨询门诊疑为Turner's征的患者取前臂内侧皮肤
liupeizc 请问哪位高手知道成纤维细胞的生长周期啊，更确切的是血管外膜成纤维细胞生长周期，谢谢！ zhujoker 估计都没人做过，你如果需要观察其生物学功能，就自己做一次，也算原创了啊！ freecell 这里有： http://www.currentprotocols.com/protocol/cb0201 本文由丁香园论坛提供，想了解更多有用的、有意
相关专题 微生物 基础培养基是人工配制的用于培养微生物的一个混合营养制品，基础培养基中含有维持微生物正常生长的营养物质，此培养基的PH值一般为7.2-7.6。某些特殊的微生物需要的PH可能偏酸或偏碱性。 培养基是根据微生物 生长繁殖时对营养物质的需要而配制的营养制品，含有营养物质及适宜的酸碱度，经灭菌后使用。 常用的培养基按用途分为：基础培养基、营养培养基、鉴别培养基、选择培养基及厌氧培养基。按物理
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