This basal culture medium contains: • Essential and non-essential amino acids • Vitamins • Other organic compounds • Trace minerals • Inorganic salts. Human Fibroblast Expansion Basal Medium does NOT contain: • Antibiotics • Antimycotics • Hormones • Growth factors • Proteins This medium is HEPES and bicarbonate buffered and is designed for use in an incubator with an atmosphere of 5% CO2/95% air. To support the plating and long-term proliferation of human dermal fibroblasts, Human Fibroblast Expansion Basal Medium must be supplemented with Low Serum Growth Supplement (LSGS, Cat. No. S-003-10), or Low Serum Growth Supplement Kit (LSGS Kit, Cat. No. S-003-K).