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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
PM-m (Pericyte Medium-m)
- 规格:
500ml/T
|
货号 |
1231 |
|
产地 |
北美 |
|
缩写 |
PM-m |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
Pericyte Medium-mouse (PM-m), when used with Pericyte Growth Supplement (PGS-m, Cat #1282) and 10mL FBS is a complete medium designed for optimal growth of normal mouse pericytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (2%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mouse pericytes in vitro.
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
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文献和实验
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
见的污染细胞培养的支原体菌群,但能够污染细胞的支原体种类是很多的,国外调查证明,大约有二十多种支原体能污染细胞,有的细胞株可以同时污染两种以上的支原体。 ??支原体污染的来源包括工作环境的污染、操作者本身的污染(一些支原体在人体是正常菌群)、培养基的污染、污染支原体的细胞造成的交叉污染、实验器材带来的污染和用来制备细胞的原始组织或器官的污染。 ??组织细胞培养工作中,主要从以下几个方面来预防支原体的污染:控制环境污染;严格实验操作;细胞培养基和器材要保证无菌;在细胞培养基中加入适量的抗生素。支原体污染
(+) 的小鼠骨髓衍生的巨噬细胞的细胞提取物和培养基进行分析。可以看到在 LPS + 尼日利亚菌素处理的细胞培养基组别中,活化的 IL-1β 信号最强。对于不同研究背景及样本的情况下,研究者需要综合考虑众多因素如:细胞类型、种属、样本来源、PAMPs 和 DAMPs 的浓度、刺激的种类及时长、细胞微环境、温度和 pH 值、氧化还原平衡、渗透压、培养条件及时间,以便找到最合理有效的刺激条件。图 6. 不同条件组合刺激小鼠 BMDM 细胞后检测的活化的 IL-1β 表达要点:通常条件下 LPS+ATP
Cell Stem Cell:潘欣 / 李爱玲团队发现胶质母细胞瘤干细胞分泌组胺,促进肿瘤进程
样本时发现,高表达 HDC 的 GSCs 周边常伴有血管的富集,且 GBM 表达的 HDC 水平越高,其血管结构越丰富,患者的生存预后越差。 为了探究 GSCs 分泌的组胺是否通过促进血管新生来促进 GBM 进程,研究人员利用基因编辑手段敲除 GSCs 中的 HDC,发现 HDC 敲除抑制了 GSCs 组胺的合成与分泌,显著抑制小鼠原位移植瘤的血管生成,并阻碍 GBM 的进程。此外,转录组测序和一系列体内体外研究证实,GSCs 分泌的组胺作用于血管内皮细胞的组胺 I 型受体(H1R),通过激活一条
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