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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
CerEpiCM (Cervical Epithelial Cell Medium)
- 规格:
500ml/T
|
货号 |
7061 |
|
产地 |
美国 |
|
缩写 |
CerEpiCM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
干冰 |
Cervical Epithelial Cell Medium (CerEpiCM), when used with Cervical Epithelial Cell Growth Supplement (CerEpiCGS, Cat #7062), is a complete medium designed for optimal growth of normal cervical epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal cervical epithelial cells in vitro.
22.) Voigt J, Christensen J, Shastri VP. (2014) "Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae."?ProcNatlAcad Sci. 111: 2942-47.
23.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) "Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization."?BMC biotechnol. 14: 17.
24.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) "Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction."?Oxid med cell longev. 2014: 904834.
25.) Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. (2014) "Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis."?PloS one. 9: e88243.
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文献和实验
22.) Voigt J, Christensen J, Shastri VP. (2014) "Differential uptake of nanoparticles by endothelial cells through polyelectrolytes with affinity for caveolae."?ProcNatlAcad Sci. 111: 2942-47.
23.) Wang Q, Yang J, Tang K, Luo L, Wang L, Tian L, Jiang Y, Feng J, Li Y, Shen B, Ly M, Huang Y. (2014) "Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization."?BMC biotechnol. 14: 17.
24.) Xing S, Yang X, Li W, Bian F, Wu D, Chi J, Xu G, Zhang Y, Jin S. (2014) "Salidroside Stimulates Mitochondrial Biogenesis and Protects against H 2 O 2-Induced Endothelial Dysfunction."?Oxid med cell longev. 2014: 904834.
25.) Xu Q, Xia P, Li X, Wang W, Liu Z, Gao X. (2014) "Tetramethylpyrazine Ameliorates High Glucose-Induced Endothelial Dysfunction by Increasing Mitochondrial Biogenesis."?PloS one. 9: e88243.
% 以上时收集细胞上清液。 解决方案 2:采用的是培养基逐步替换的方法。逐步减少原干细胞培养基的用量和逐步增加外泌体专用间充质干细胞培养基的用量,在干细胞融合度 20% 时,吸取一半的原始培养基,加入一半的外泌体专用间充质干细胞培养基,细胞培养 24 小时后,再替换为完全的外泌体专用间充质干细胞培养基的进行培养。 20、关于外泌体红色荧光标记染料(PKH26/PKH67)相关实验注意事项 a、建议用于染料标记的外泌体原始浓度达到 0.5~1μg/μL。外泌体浓度过低实验失败风险较高。 b、外泌
性,一般与激素或其它物质起相互协同或加成作用。另一种常见的添加物就是血清替代物,目前已有许多可完全或部分替代传统培养液中血清成分的商业产品,其稳定性较好,但批次间仍有差别,产品的成分也不很确定。2、细胞培养基配制 2.1 干粉培养基原倍液的配制 1)配制过滤除菌的细胞培养基 1)阅读培养基使用说明,确定需要添加何种添加剂(如NaHCO3、L-谷氨酰胺、丙酮酸钠、HEPES等)。 2)根据所需将培养基全部倒入一容器中,用少量注射用水(20℃~30℃)将袋内残留培养基洗下,并入容器。加注射用水至总体
相关专题 细胞培养基的配制 millipore .html' target='_blank'>Millipore 公司宣布独家推出表皮角质形成细胞的3D细胞培养 基。该培养基由CELLnTEC公司(CELLnTEC Advanced Cell Systems)研发。根据两家公司在2006年签订的合作协议,millipore .html' target='_blank'>Millipore 共同拥有并出售。 过去,3D
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