相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
CMM-sf (Cardiac Myocyte Medium-serum free)
- 规格:
500ml/T
|
货号 |
6101 |
|
产地 |
北美 |
|
缩写 |
CMM-sf |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
干冰 |
Cardiac Myocyte Medium-serum free (CMM-sf), when used with Cardiac Myocyte Growth Supplement-serum free (CMGS-sf, Cat #6152) is a complete medium designed for the culture of normal cardiac myocytes in serum-free conditions. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the culture of normal cardiac myocytes in vitro under serum free conditions.
15.) AbuBakar S, Shu MH, Johari J, Wong PF. (2014) "Senescence Affects Endothelial Cells Susceptibility to Dengue Virus Infection."?Int j med sci. 11: 538.
16.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) "Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains."?Infect immune. 82: 949-59.
17.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells."?Int j mol sci. 15: 484-503.
18.) Lim PJ, Chu JJH. (2014) "A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells."?PLoSnegl trop dis. 8: e2661.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验
15.) AbuBakar S, Shu MH, Johari J, Wong PF. (2014) "Senescence Affects Endothelial Cells Susceptibility to Dengue Virus Infection."?Int j med sci. 11: 538.
16.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) "Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains."?Infect immune. 82: 949-59.
17.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells."?Int j mol sci. 15: 484-503.
18.) Lim PJ, Chu JJH. (2014) "A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells."?PLoSnegl trop dis. 8: e2661.
相关专题 无血清培养基产品选择指南 &NBS p; 无血清细胞培养 基 天然培养基 优 点 更利于细胞生长;性能更加一致 ;容易进行纯化和下游加工 ;细胞功能的精确评估 ;增强生长和/或产量 ;生理反应性的较好对照 ;增强细胞内中介物的检测 ;可以消除来自血清的不均
细胞来说要少很多。 最后,针对细胞上清液的外泌体的提取。这个是老生常谈的问题,这里就不再多讲了。如果实验室有超速离心机的话,那就使用超速离心的方法,按照前文建议的预处理操作后,超离后的沉淀也会增加的,同时外泌体的质量也会提升。如果实验室没有超离的情况下,也可以通过试剂盒的方法来提取,除了进口品牌外,也可以使用一些国产试剂盒,质量也不亚于进口品牌的。 总结一下,为了获得高质量的外泌体:需要在细胞培养时减少正常血清的使用,推荐去外泌体血清或者外泌体专用无血清培养基;收获
病毒繁殖,而且可能导致口蹄疫病毒在抗体压力下选择性变异。牛血清中潜在污染朊病毒或疯牛病病毒可给动物疫苗带来潜在的生物危害,TPB或LAH等动物来源成份也存在安全隐患。为提升产品质量及安全性,目前国外多数口蹄疫疫苗生产厂家急于将现使用的含血清生产工艺升级为不含血清生产工艺。 自从20世纪的80年代起,细胞培养基(液)发展迅速,低血清细胞培养基、无血清细胞培养基、无血清无动物来源成分细胞培养基已商品化,为疫苗生产中少使用或不使用血清奠定了物质基础。1.2 国内应用现状 默克密理博北京清大天一
技术资料暂无技术资料 索取技术资料






