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- Sciencell
- A-R1811-sf
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
RACM-sf（Rat Astrocyte Conditioned Medium-serum free）,100ml
- 规格：
100ml/T
|
货号 |
R1811-sf |
|
产地 |
美国 |
|
缩写 |
RACM-sf |
|
规格 |
100 ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
Astrocytes are the most abundant cells in the central nervous system (CNS). Astrocytes provide a variety of biological functions, such as supporting endothelial cells involved in blood-brain barrier formation, providing nutrients to neurons, maintaining extracellular ion balance, and promoting repair in the CNS. Astrocyte conditioned medium-serum free (ACM-sf) has been widely used to support the growth of neurons and endothelial cells in culture. ACM has also been shown to protect neurons from corticosterone-induced damage .
Rat ACM-sf is a liquid medium prepared from primary rat astrocyte cultures by using astrocyte medium (Cat #1801) without serum and conditioned for 48 hours. The medium is sterile filtered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
26.) Yang J, Wang Q, Qiao C, Lin Z, Li X, Huang Y, Zhou T, Li Y, Shen B, Lv M, Feng J. (2014) "Potent anti-angiogenesis and anti-tumor activity of a novel human anti-VEGF antibody, MIL60."?Cell molimmunol. 11: 285-93.
27.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) "In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications."?PloS one. 9: e98674.
28.) Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z. (2014) "A Dual-Role of Gu-4 in Suppressing HMGB1 Secretion and Blocking HMGB1 Pro-Inflammatory Activity during Inflammation."?PloS one. 9: e89634.
29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.
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文献和实验
26.) Yang J, Wang Q, Qiao C, Lin Z, Li X, Huang Y, Zhou T, Li Y, Shen B, Lv M, Feng J. (2014) "Potent anti-angiogenesis and anti-tumor activity of a novel human anti-VEGF antibody, MIL60."?Cell molimmunol. 11: 285-93.
27.) Zhao N, Watson N, Xu Z, Chen Y, Waterman J, Sankar J, Zhu D. (2014) "In Vitro Biocompatibility and Endothelialization of Novel Magnesium-Rare Earth Alloys for Improved Stent Applications."?PloS one. 9: e98674.
28.) Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z. (2014) "A Dual-Role of Gu-4 in Suppressing HMGB1 Secretion and Blocking HMGB1 Pro-Inflammatory Activity during Inflammation."?PloS one. 9: e89634.
29.) Jandu SK, Webb AK, Pak A, Sevinc B, Nyhan D, Belkin AM, Flavahan NA, Berkowitz DE, Santhanam L. (2013) Nitric oxide regulates tissue transglutaminase localization and function in the vasculature.?Amino Acids. 44: 261-9.
【求助】请教！我用无血清培养基养的胃癌原代细胞，为什么会出现这种小颗粒？
暮色英雄 我是用无血清NB培养基+EGF+bFGF+B27+p/s双抗的培养基培养的胃癌原代细胞，培养基已经用0.22μm过滤器过滤除菌，但是培养细胞之后出现很多小颗粒，不知道是什么东西，这照片是昨天照的，今天再观察的时候这些小颗粒已经长满了，我师姐帮我看了说是污染，然后我把细胞倒掉了，但是她也不知道这是什么污染。希望各位大侠能帮小弟看看这到底是不是污染，是什么东西？谢谢了 这是20x镜拍
后，转移细胞悬液至新瓶中，加入DMEM/F12完全培养基，在37ºC, 5%CO2培养箱中培养9天（每3天更换培养液一次），第9天将培养瓶置于恒温旋转摇床上37ºC, 240rpm，摇18小时，去掉悬浮的细胞，用D-Hanks液清洗3次，进行传代培养即得纯化的星形胶质细胞。经免疫细胞化学法检测，98%以上的细胞GFAP免疫反应为阳性。
机械性损伤导致细胞死亡等缺点。本实验采用视神经组织块培养的方法，对新生大鼠的视神经胶质细胞进行条件化原代培养，利用少突胶质细胞和星形胶质细胞生长条件的不同，采用条件培养基纯化2种细胞。少突胶质细胞和Ⅱ型星形胶质细胞是从一种普通双潜能的少突胶质细胞－2型星形胶质细胞祖细胞（oligodendrocyte-type-2astrocyte progenitor cell，O2A）发育而来。大鼠出生后约1周，少突胶质细胞逐渐成熟。因此，从新生大鼠取材可获得O2A祖细胞。体外研究发现甲状腺素、胰岛素、转铁
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