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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Human EIF4B Protein, N-GST&C-His
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug

| Product name | Recombinant Human EIF4B Protein, N-GST&C-His |
|---|---|
| Catalog No. | HB606022 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | P23588 |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Alternative Names | Eukaryotic translation initiation factor 4B; eIF-4B; EIF4B |
| Species | Homo sapiens (Human) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003...
Product categories and application fields.
Main products...
Application field: Infectious disease research, Neuroscience, Immunology, Oncology
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文献和实验• eIF4B mRNA Translation Contributes to Cleavage Dynamics in Early Sea Urchin Embryos., PMID:36290313
• Deficiency of eIF4B Increases Mouse Mortality and Impairs Antiviral Immunity., PMID:34566982
• eIF4B enhances ATF4 expression and contributes to cellular adaptation to asparagine limitation in BRAF-mutated A375 melanoma., PMID:34403810
• Disordered regions of human eIF4B orchestrate a dynamic self-association landscape., PMID:39384813
• [Effect of eIF4B knockout on apoptosis of mouse fetal liver cells]., PMID:36151816
• Eukaryotic initiation factors eIF4F and eIF4B promote translation termination upon closed-loop formation., PMID:40066881
• Elaiophylin targets EIF4B to suppress the growth of esophageal squamous cell carcinoma via the PI3K/AKT signaling pathway., PMID:39694222
• Autophagy induction promoted by m6A reader YTHDF3 through translation upregulation of FOXO3 mRNA., PMID:36195598
• eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology., PMID:28874824
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, A., Sopko, R., McCartney, R.R., Schmidt, M.C., Rachidi, N., Lee, S.J., Mah, A.S., Meng, L., Stark, M.J., Stern, D.F., De Virgilio, C., Tyers, M., Andrews, B., Gerstein, M., Schweitzer, B., Predki, P.F., and Snyder, M. 2005. Global analysis of protein
如下:几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利:N-端,C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合,你可以将目的基因替换MCS―Intein2―几丁质结合蛋白,如果想做C端融合表达,就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS,不用分2个载体;而且做过克隆的人会很有体会:很多时候双酶切载体,由于2个酶切位点都多克隆位点上,没有小片断切下来,所以是否成功双酶切就不好判断,如果用Twin载体就会切下一个片断
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