重组人Glypican 3/GPC3蛋白(Site-Specific AF 647 Conjugation, His Tag)

重组人Glypican 3/GPC3蛋白（产品说明）
蛋白名称：Human Glypican 3/GPC3 Protein (Site-Specific AF 647 Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human GPC3 (P51654-1) (Met 1-His 559) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
表达宿主：HEK293 Cells
蛋白活性：1.Flow cytometric analysis of anti-Glypican 3 CAR expression. 5e5 of anti-Glypican 3 CAR-293 cells were stained with 100 μL of 1 μg/mL of AF 647-conjugated human Glypican 3 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10088-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 647 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 647-conjugated Glypican 3 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 647-conjugated human Glypican 3 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10088-H86H-SG) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 25 & Ser 359
蛋白分子量：The recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 64 kDa, Cleaved partially by a furin-like convertase to N and C-terminal fragment with predicted molecular mass of 38 KDa and 26 kDa.
蛋白NP号：P51654-1
蛋白氨基酸序列：Met1-His559