- ￥4890 - 26170
- Sino Biological
- 北京
- 10429-HNAH-UE
- 2026年05月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human / Mouse / Rat / Cynomolgus / Rhesus Activin A / INHBA Protein, Endotoxin-Free
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg/1.00 mg
| 规格： | 100.00 µg | 产品价格： | ￥4890.0 |
|---|---|---|---|
| 规格： | 1.00 mg | 产品价格： | ￥26170.0 |
蛋白名称：重组人/Mouse/Rat/食蟹猴/Rhesus Activin A/INHBA蛋白
蛋白构建：A DNA sequence encoding mature form of human / mouse / rat / cynomolgus / rhesus INHBA (NP_002183.1) (Gly311-Ser426) was expressed. The mature form sequences of the human, mouse, rat, cynomolgus and rhesus INHBA are identical.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
蛋白活性：1.Refer to: Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is 0.5-5ng/mL. 2.Refer to: Loaded Recombinant Rabbit ACVR2B Protein, His Tag (Cat. No. 65178-T08H) on HIS1K Biosensor, can bind Recombinant Human / Mouse / Rat / Cynomolgus / Rhesus/ Rabbit Activin A Protein (Cat. No. 10429-HNAH) with an affinity constant of 0.182 nM as determined in BLI assay (Sartorius Octet RED384) . 3.Refer to: Recombinant Rabbit ACVR2B Protein, His Tag (Cat. No. 65178-T08H) captured on NTA chip, can bind Recombinant Human, Mouse, Rat, Cynomolgus, Rhesus Activin A Protein (Cat. No. 10429-HNAH) with an affinity constant of 6.770 pM as determined in an SPR assay (Biacore 8K) . 4.Refer to: Immobilized Recombinant Human/Mouse/Rat/Cynomolgus/Rhesus Activin A/INHBA Protein(Cat 10429-HNAH) at 2 μg/mL (100 μL/well) can bind Recombinant Human ACVR2B / ActivinR-IIB Protein (His Tag), Biotinylated(Cat: 10229-H08H-B) , the EC50 is 2-8ng/mL .
蛋白内毒素：< 0.5 EU per mg protein.
预测N端：Gly 311
蛋白分子量：The recombinant human / mouse / rat / cynomolgus / rhesus INHBA consists of 116 amino acids and predicts a molecular mass of 13 kDa. As a result of glycosylation, it migrates as an approximately 16-18 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 19.4 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号：NP_002183.1
蛋白氨基酸序列：Gly311-Ser426
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文献和实验下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
扫描仪中，都采用机械式的二维X，Y线性扫描技术实现，即X，Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置，由于驱动系统的频率限制，驱动器的扫描惯性大，使得扫描效率低，分析时间相当长；并且往复行程长，对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描，我们的实验装置设计如图2，采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描，Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中，对于f-è物镜，满足x＝2fè（è为振镜的摆动角度，f为物镜焦距）的线性
Analysis of RB Action in DNA Damage Checkpoint Response
checkpoint response: (1) transcriptional repression of E2F-regulated genes (cyclin A reporter assay); (2) induction of cell cycle arrest (Brd-U incorporation assay); and (3) inhibition of DNA double-strand break accumulation (phosphorylated-histone H2A.X
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