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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human / Cynomolgus / Rhesus CD28 Protein (ECD, His Tag), Endotoxin-Free
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg/1.00 mg
| 规格: | 100.00 µg | 产品价格: | ¥3490.0 |
|---|---|---|---|
| 规格: | 1.00 mg | 产品价格: | ¥26170.0 |
蛋白名称:重组人/ 食蟹猴/ Rhesus CD28蛋白(胞外区, His标签)
蛋白构建:A DNA sequence encoding the human/cynomolgus/rhesus CD28 (P10747-1 & Q0PDN3-1 & NP_001036106.2) (Asn19-Pro152) was expressed with a polyhistidine tag at the C-terminus and a signal peptide (Q0PDN3-1/NP_001036106.2, Met1-Gly18) at the N-terminus. Human, Cynomolgus and Rhesus CD28 sequences (Asn19-Pro152) are identical.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:1.Refer to: Immobilized Recombinant Human / Cynomolgus / Rhesus CD28 Protein (ECD, His Tag) (Cat: 90182-C08H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus CD80 / B7-1 Protein (Fc Tag) (Cat: 90268-C02H), the EC50 is 150-600 ng/mL. 2.Refer to: Loaded Recombinant Human B7-1/CD80 Protein, hFc Tag (Cat. No. 10698-H02H) on proA Biosensor, can bind Recombinant Human, Cynomolgus, Rhesus CD28 Protein, His Tag (Cat. No. 90182-C08H) with an affinity constant of 0.895 μM as determined in BLI assay (Sartorius Octet RED384) .
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Asn 19
蛋白分子量:The recombinant Human/cynomolgus/rhesus CD28 consists of 145 amino acids and predicts a molecular mass of 16.6 kDa. As a result of glycosylation, the band migrates approximately 40-70 kDa in the SDS-PAGE under reducing conditions.
蛋白NP号:P10747-1 & Q0PDN3-1 & NP_001036106.2
蛋白氨基酸序列:Met1-Pro152
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