- ￥6640 - 27370
- Sino Biological
- 北京
- 10108-H02H-UE
- 2026年05月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag), Endotoxin-Free
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg/1.00 mg
| 规格： | 100.00 µg | 产品价格： | ￥6640.0 |
|---|---|---|---|
| 规格： | 1.00 mg | 产品价格： | ￥27370.0 |
蛋白名称：重组人ACE2 / Angiotensin-Converting Enzyme 2蛋白(Fc标签)
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Ser 740) of human ACE2 precursor (NP_068576.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性：1.Loaded Recombinant SARS-CoV-2 Spike RBD-mFc Recombinant Protein (HPLC-verified) (Cat. No. 40592-V05H) on AMC Biosensor, can bind Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat. No. 10108-H02H-UE) with an affinity constant of 1.36 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 2.Recombinant Human ACE2 / Angiotensin-Converting Enzyme 2 Protein (Fc Tag) (Cat: 10108-H02H-UE) captured on Protein A chip, can bind Recombinant SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (His Tag) (Cat: 40591-V08H41) with an affinity constant of 9.621 nM as determined in an SPR assay (Biacore T200) (Routinely tested).
蛋白内毒素：< 0.5 EU per mg protein.
预测N端：Gln 18
蛋白分子量：The recombinant human ACE2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 961 amino acids and predicts a molecular mass of 110.3 kDa. As a result of glycosylation, the band migrates approximately 140-150 kDa in the SDS-PAGE under reducing conditions.
蛋白NP号：NP_068576.1
蛋白氨基酸序列：Met 1-Ser 740
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文献和实验Severe acute respiratory syndrome (SARS) coronavirus (CoV) contains a spike (S) protein that binds to a receptor molecule (angiotensin-converting enzyme 2; ACE2), induces membrane fusion, and serves as a neutralizing epitope. To study
to dephosphorylate Hxk2p in vivo and that the peptide motif (K/R)(X)(I/V)XF is necessary for its PP-1 targeting function. These studies therefore demonstrate how a proteomics approach can be used to first identify enzyme targets in cells and then direct
，这些蛋白质可以是高度多样性的。当前用来产生这些合成库的技术包括饱和突变（saturation metagenesis)；利用 PCR 的随机突变；“基因混编”，即将类似来源的 DNA 片段组合在一起；“定向”突变，即改变特定区域的 DNA 编码；以及序列的非同源、随机重组策略等（非同源重组可参见文献 [1]、[2] 及 [9])。对于设计合意的合成库的大小，下列考虑是必需的。 对于小的多肽库（如 10 个氨基酸长的十肽），有 2010 ( ≈ 1.02X1013，原文错误——译者注）种可能的氨
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